Dynamics of Calcium during In vitro Microspore Embryogenesis and In vivo Microspore Development in Brassica napus and Solanum melongena
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چکیده
منابع مشابه
Dynamics of Calcium during In vitro Microspore Embryogenesis and In vivo Microspore Development in Brassica napus and Solanum melongena
Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. ...
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Isolated microspores of Brassica napus are developmentally programmed to form gametes; however, microspores can be reprogrammed through stress treatments to undergo appropriate divisions and form embryos. We are interested in the identification and isolation of factors and genes associated with the induction and establishment of embryogenesis in isolated microspores. Standard and normalized cDN...
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Brassica napus cultivar Westar is non-embryogenic under all standard protocols for induction of microspore embryogenesis; however, the rare embryos produced in Westar microspore cultures, induced with added brassinosteroids, were found to develop into heritably stable embryogenic lines after chromosome doubling. One of the Westar-derived doubled haploid (DH) lines, DH-2, produced up to 30% the ...
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چکیده ندارد.
15 صفحه اولEffects of gibberellin, abscisic acid and embryo desiccation on normal plantlet regeneration, secondary embryogenesis and callogenesis in microspore culture of Brassica napus L. cv. PF704
Most of the microspore-derived embryos can not regenerate normally in rapeseed. The effects of gibberellins (GA3), abscisic acid (ABA), and embryo desiccation on normal plantlet regeneration were studied. The donor plants were grown in a growth chamber at day/night temperatures of 15/10˚C with a 16/8h photoperiod, respectively. Microspores were isolated from whole buds of 2.5-3.5mm in length, c...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2017
ISSN: 1664-462X
DOI: 10.3389/fpls.2017.01177